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KMID : 0357920010350020129
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Korean Journal of Pathology
2001 Volume.35 No. 2 p.129 ~ p.136
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A Study on the Expression of Proliferating Cell Nuclear Antigen and Apoptosis of theHepatocellular Carcinoma in Human and Hepatitis B Virus X Transgenic Mice
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Moon Hyung-Bae
Yu Dae-Yeul
Yoo Hyung-Ryun
So Byung-Joon
Chae Kwon-Mook
Kim Haak-Cheol
Yun Ki-Jung
Han Won-Cheol
Jo Hyang-Jeong
Kim Bo-Yong
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Abstract
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Background: This experiment was designed to study the cell kinetics of hepatocellular carcinoma (HCC) in both hepatitis B virus X (HBx) transgenic mice and humans.
Methods: The immunohistochemical stain of proliferating cell nuclear antigen (PCNA) and TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay of apoptosis were used on formalin fixed-paraffin embedded tissues.
Results: PCNA labeling indices (PCNA-LI) in the liver of HBx transgenic mice were markedly increased in HCC (11.3%) compare to the dysplastic areas (1.3%) and in the liver of non-transgenic littermates (0.1%). There was no significant difference of PCNA-LI in the dysplastic areas between HCC developed mice and non-HCC developed mice. Apoptosis labeling indices (Apoptosis-LI) in both the dysplastic areas and HCC of HBx transgenic mice were similar to those of non-transgenic littermates. PCNA-LI was markedly increased in human HCC (28.9%) compare to the background of HCC (2.9%) and the control liver (2.9%). Apoptosis-LI was decreased in human HCC (0.3%) compare to the background of HCC (0.4%) and the control liver (1.0%).
Conclusion: There is a marked increase of cell proliferating activity in human HCC and in HCC of HBx transgenic mice, and there is a decrease of apoptosis in human HCC, but not in HCC of HBx transgenic mice.
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KEYWORD
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Transgenic, Hepatocellular, Carcinoma, Proliferating cell nuclear antigen, Apoptosis
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